Use this url to cite publication: https://hdl.handle.net/20.500.12512/116215
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Evaluation of the Antioxidant Effects of Aronia, Pomegranate and Elderberry on the Brains of Laboratory Mice / A. Žentelytė, R. Bernotienė
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Evaluation of the Antioxidant Effects of Aronia, Pomegranate and Elderberry on the Brains of Laboratory Mice / A. Žentelytė, R. Bernotienė
Publisher (trusted)
Vilnius University Press |
Date Issued
Date Issued |
---|
2022-11-25 |
Extent
p. 58-58.
Is part of
14th International Conference of the Lithuanian Neuroscience Association : 25 November 2022, Vilnius, Lithuania : Abstract book / Lithuanian Neuroscience Association. Vilnius University. Vilnius : Vilnius University Press, 2022. ISBN 9786090707968.
Version
Originalus / Original
Description
no. P43
Poster presentations
ISBN 978-609-07-0796-8
Field of Science
Abstract
Antioxidants are compounds that help protect the cells of your body from damage caused by free radicals. Free radicals are always present in your body, but having too many of them can be harmful and contribute to a number of chronic diseases. Aronia, pomegranate and elederberry are rich in antioxidants and polyphenolic compounds that offer protection from this damage. The antioxidant properties of various berries are measured by the polyphenol content each has. Polyphenol content is the marker for bioactivity of antioxidants. So, getting antioxidants from berries and fruits such as aronia, pomegranate and elderberry is a great way to support overall health and help prevent against the activity of radical oxygen species which would otherwise cause long-term damage. This study was performed to evaluate the antioxidant effects of aronia, pomegranate and elderberry extracts on the brains of laboratory mice. Our experiments were performed on outbred white laboratory mice by changing drinking water with plant extract solutions of aronia, pomegranate and elderberry. Exposure time was 21 days. Lipid peroxidation level was estimated spectrophotometrically by measuring the concentration of MDA produced by reaction with TBA at 535 nm and 520 nm light wavelengths. The concentration of antioxidant GSH was measured spectrophotometrically by reaction with DTNB to give compound TNB, which absorbs light wavelength at 412 nm. GSH is important in protecting cells against damage from radiation, free oxygen radicals, heat, and sulfhydryl reactive agents, and provides the bulk of sulfhydryl groups for the detoxication of electrophilic xenobiotics. So, in our experiments we showed that after 21 days of exposure to aronia, pomegranate and elderberry, the content of GSH was increased by 281 % (p<0.001), 302 % (p<0.0001) and 297 % (p<0.0001), respectively, as compared to control mice group. In further experiments we determined the content of MDA in mice brain. It is very important, becouse determining the level of MDA is usually the most practical and reliable method for detecting and screening oxidative stress. Our results showed that after treatment with aronia, pomegranate and elderberry, the content of MDA was significantly increased by 50 % (p<0.001) in all treated groups, as compared to control mice group. Our experiments showed that extracts of aronia, pomegranate and elderberry increased antioxidant GSH level, but unfortunately did not protect lipids from peroxidation.
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9786090707968
Other Identifier(s)
(LSMU ALMA)991680983207106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)