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T Cell Phenotypes and their Relation to Vitamin D Level in Atopic Diseases / Daina Bastyte, Laura Tamasauskiene, Dovydas Bagdonas, Brigita Sitkauskiene
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
T Cell Phenotypes and their Relation to Vitamin D Level in Atopic Diseases / Daina Bastyte, Laura Tamasauskiene, Dovydas Bagdonas, Brigita Sitkauskiene
Publisher (trusted)
European Society for Clinical Cell Analysis (ESCCA) |
Date Issued
Date Issued |
---|
2022-09-21 |
Extent
p. 42-43.
Is part of
ESCCA 2022 congress : abstracts : Belfast, Northern Ireland, September 21-24, 2022 / European Society for Clinical Cell Analysis (ESCCA). Amsterdam : European Society for Clinical Cell Analysis (ESCCA), 2022.
Version
Originalus / Original
Description
no. IMM-03
Posters. Immunology (IMM)
Field of Science
Abstract
Introduction: Immune response in atopic diseases could be associated with different T cell phenotypes. Moreover, vitamin D may have a relation with T cells immune response. The relationship between T cell phenotypes and vitamin D in atopy has not well-defined. The aim of the study was to evaluate T cell phenotypes and their possible relation with vitamin D level in subjects with atopy. Methods: In total, 30 subjects with atopy (15 with mild to moderate atopic dermatitis – AD, 15 with mild to moderate allergic asthma – AA) and 15 age-matched healthy subjects who do not take vitamin D supplements were involved in the study. Peripheral blood mononuclear cells were stained with monoclonal antibodies (anti-CD25–CD4–FoxP3 for Treg, anti-CD4–IL-17A–INF-GMA–IL-4 for Th1/Th2/Th17 cells) and evaluated using flow cytometry. Serum 25-hydroxy vitamin D level was evaluated by ELISA. According to the recomendations, subjects were divided into groups based on their vitamin D level: severe deficiency < 20 nmol/l, deficiency 20-50 nmol/l, insufficiency 50-75 nmol/l, normal amount 75-200 nmol/l. Results: Significantly lower percentages of Treg cells were detected in subject with AA compared to AD or control groups (1.56 ± 0.15 vs 2.34 ± 0.26 and 2.35 ± 0.16 %; respectively, p< 0.05). In subject with AD, relative numbers of Th17 cells were higher than in control group (0.96 ± 0.15 vs 0.54 ± 0.06 %; p< 0.05). AD and AA groups had significantly higher proportion of Treg and Th17 cells compared to control group (0.49 ± 0.09 and 0.47 ± 0.08 vs 0.25 ± 0.03; respectively, p< 0.05). In subjects with atopy there was a positive correlation between Th2 and Th1 cells (r=0.57, p=0.001), Th2 and Th17 cells (r=0.37, p< 0.05). Negative significant correlation was established between Th1/Th2 proportion and vitamin D level in subjects with atopy (r=-0.38, p< 0.05). There was no significant difference of vitamin D levels between the groups. In subject with atopy and vitamin D severe deficiency were detected significant higher proportion of Th1/Th2 cells compared to the groups of atopic subject with vitamin D deficiency, insufficiency or normal amount (0.45 ± 0.25 vs. 0.19 ± 0.16; 0.14 ± 0.5; 0.21 ± 0.3; respectively, p< 0.05). Conclusions: Decrease of Treg and increase of Th17 cells as well as association between Th2/Th1 cells and vitamin D in subjects with allergic asthma and/or atopic dermatitis let us hypothesize about an important role of different T cell phenotypes and vitamin D in the pathogenesis of diseases related to atopy.
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)991666987107106
Coverage Spatial
Airija / Ireland (IE)
Language
Anglų / English (en)