Use this url to cite publication: https://hdl.handle.net/20.500.12512/115648
Options
Effect of ambient temperature and humidity on blood metabolic profile and cortisol level in two different sheep breeds / Jurgita Autukaitė, Indrė Poškienė, Vida Juozaitienė, Henrikas Žilinskas
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
Effect of ambient temperature and humidity on blood metabolic profile and cortisol level in two different sheep breeds / Jurgita Autukaitė, Indrė Poškienė, Vida Juozaitienė, Henrikas Žilinskas
Publisher (trusted)
The National Association of Spanish Specialists in Bovine Medicine (ANEMBE) |
Date Issued
Date Issued |
---|
2022-09-04 |
Extent
p. 294-294.
Is part of
31st World Buiatrics Congress (WBC 2022) : September 4th to 8th, 2022, Madrid, Spain : Abstract Book : Volume 2 / World Association for Buiatrics (WAB). The National Association of Spanish Specialists in Bovine Medicine (ANEMBE). Madrid : The National Association of Spanish Specialists in Bovine Medicine (ANEMBE), 2022.
Version
Originalus / Original
Description
Field of Science
Abstract
Objectives: Climatic variations directly affect the animals, changing their physiology. Quantitative and morphological changes in blood cells are associated with heat stress. Ap-parently in sheep, hyperthermia conditions during prolonged periods reduce blood metabolite levels related to the energetic metabolism and increase metabolite concentrations. The aim of the study were to determine the effect of temperature and humidity index (THI) and breed on biochemical blood parame-ters and cortisol level.Materials and methods: This experiment was conducted from 2018 December till 2019 December. Two sheep breeds from two different farms were chosen for this study: Merino (n=19), Lithuanian blackhead (n=19).For the study sheep (n=38) were selected according to those: adult female sheep (3 years old), not pregnant during the whole investigation, clin-ical healthy and without any signs of diseases after a precise clinical examination. Means of body weight of the animals in the groups were roughly equal (on average 35 kg). The ani-mals were kept in similar conditions in a loose housing system and were fed a feed ration throughout the year at the same time balanced according to their physiological needs. The cli-mate of the country is transitional between the maritime type of Western Europe and the continental type found farther east, characterized by warm, dry summers and fairly severe win-ters. Average ambient temperature during experimental year was 10.50 ± 1º C, relative humidity 77.60 ± 2%. Blood sam-ples were taken one time per month at 7:00 h to 8:00 h after overnight fasting from the identical animals. The values deter-mined were calcium (Ca), phosphorus (P), magnesium (Mg), copper (Cu), zinc (Zn), iron (Fe), urea, total proteins, (TP), glucose (Gl), aspartate aminotransferase (AST), gamma glu-tamyl transferase (GGT), total bilirubin (TBIL), albumin (Alb), creatinine (Crea), lactate dehydrogenase (LDH) and cortisol. According to the test date, subgroups were created by breed (2 breeds) and THI (3 classes). The daily temperature-humid-ity index (THI) values were calculated using the equation (a) by Marai et al. (2007). THI _ T-(0.31- 0.0031× RH) × (T-14.4) (a), where T is the dry-bulb temperature and RH is the relative humidity. The average daily values of temperature and relative humidity were exploited to calculate the daily values of THI that were analysed in this study. Normal distributions for all blood indicators were assessed by the Kolmogorov-Smirnov test. The results of statistical analysis were expressed as the mean±standard error. The linear relationship between bio-chemical blood indicators and cortisol were evaluated using Pearson‘s correlation. The one-way ANOVA test was used to assess the influence of breed and THI level, on blood pa-rameters. A probability of less than 0.05 was considered as being significant (P <0.05). This work followed all ethical prin-ciples involving animals in research. Study approval number – PK014606.Results: The analysis showed that the breed had a sta-tistically significant effect on many blood parameters, with the exception of the concentration of Fe, Mg, P and cortisol. The Lithuanian native breed had significantly higher levels of urea (7.48±0.32 mmol/l), Crea (108.27±1.48 μmol/l), Ca (2.57±0.02 mmol/l), TP (72.25±0.67 g/l) (P <0.001) and a lower levels of TBIL (2.23±0.05 μmol/l ) and LDH (1067.15±21.87 U/l) (P <0.001) compared to the Merino breed. Studies have shown that THI 1 prevailed in Lithuanian native breed records, THI 2 – in Merino breed. The Merino breed had 1.3 times less TI 3 records than the Lithuanian native breed. THI 1 class con-sisted of 181 records (48.66%), THI 2-160 (43.01%), and THI 3-31 records of animals (8.33%). THI levels had a statisti-cally significant effect on blood urea, Gl, Crea, TP, TBIL, Cu, Zn, cortisol (P ≤ 0.001) and LDH (P = 0.022). Blood cortisol statistically significant correlated with urea, Alb (P<0.001), P (P=0.002), Crea (P=0.034) and TP (P=0.037).Conclusions: According our results we can conclude, that the response to temperature is dependent on animal breed and enviromental conditions. The Lithuanian native breed had significantly higher levels of urea, Crea, Ca, TP (P <0.001) and a lower levels of TBIL and LDH (P <0.001) compared to the Merino breed. It was found that heat stress had a statistically significant effect on blood urea, Gl, Crea, TP, TBIL, Cu, Zn, cortisol (P ≤ 0.001) and LDH (P = 0.022).
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)991666686507106
Coverage Spatial
Ispanija / Spain (ES)
Language
Anglų / English (en)